Dengue infection is a major cause of morbidity in tropical and subtropical regions. It is a mosquito borne viral infection that may be asymptomatic or may cause undifferentiated fever, dengue fever, dengue hemorrhagic fever or dengue shock syndrome. The Dengue virus group consists of four closely related serotypes and belongs to the family Flaviviridae. Currently there is no vaccine or effective antiviral drugs to treat the infection.
Source:Dengue virus type 2 strain 16681 cultured in Vero cells.
Preparation:Dengue virus particles are concentrated from tissue culture supernatant by precipitation and ultracentrifugation. Antigen is purified by sucrose density gradient centrifugation. Virus particles are separated from the sucrose containing buffer by ultracentrifugation before resuspension in Medium 199. The antigen preparation is inactivated by room temperature incubation with formaldehyde. The formaldehyde is neutralized by the addition of sodium bisulphite. This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Source Antigen：Native
Grade：Inactivated Pathogen
Purity：Inactivated Pathogen
Form：Supplied as a liquid in Medium 199. Preservative free. Inactivated.
