Cytosolic sulfotransferases are a family of Phase II drugmetabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. They have important roles in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase watersolubility of xenobiotics and therapeutic drugs. They are distinct from Golgi resident sulfotransferases by lacking Nterminal signalanchor domains and residing only in the cytoplasm. SULT2A1 is primarily expressed in liver and adrenal tissues where it sulfates steroids and bile acids (46). It is also called DHEA/bile acid sulfotransferase and plays roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and, at the same time, preventing toxicity from bile acid accumulation.
Recombinant enzyme corresponding to Ser2-Glu285 with a Met and N-terminal 6-His tag of human Sulfotransferase 2A1 expressed in E. coli. (Q06520)
N-terminal Sequence Analysis:Met
Predicted Molecular Mass:35kD
SDS-PAGE:36kD, reducing conditions
Activity:Measured by its ability to transfer sulfate from PAPS to t-Dehydroandrosterone (DHEA).
Specific Activity:≥10 pmol/min/ug
Endotoxin:≤1EU per 1ug (LAL)
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤1.0EU/ug (LAL)
Form：Supplied as a liquid in 25mM Tris, 150mM sodium chloride, pH 7.5, 1mM DTT
