Human ciliary neutotrophic factor (CTNF), is a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy.
Source:Recombinant corresponding to human CTNF, 199aa, expressed in E. coli.
Molecular Weight:~22.7kD
Endotoxin:≤1EU/ug
Activity:The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml.
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Functional Assays: 50ng/ml-150ng/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 200ul of 5mM sodium phosphate, pH 7.5. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, expressed in E. coli
Grade：Highly Purified
Purity：~98% (SDS PAGE/HPLC)
Form：Supplied as a lyophilized powder in 5mM sodium phosphate, 75mM sodium chloride. Preservative free. Reconstitute with 200ul of 5mM sodium phosphate, pH 7.5.
