Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Molecular Weight:~129kD (sedimentation equilibrium with and without 6 M guanidine hydrochloride) and ~140kD (SDS gel electrophoresis and gel filtration).
Activity:~10U/ml
Specific Activity:4.5U/mg (may vary from lot to lot)
Unit Definition of Activity:One unit will produce 1.0 micromole of hippuric acid from Hippuryl-His-Leu per minute in 50mM HEPES and 300 mM NaCl at pH 8.3 and 37°C.
Km:2.6mM using Hippuryl-L-histidyl-L-leucine as the substrate
Storage and Stability:Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：0.25U
Source Antigen：Rabbit lung
Grade：Purified
Purity：Purified
Form：Supplied as liquid. No preservative or stabilizer added.
