Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum- localized-2 (Noelin-2) i s a 50kD (predicted), secreted member of the olfactomedin/noelin family of proteins. Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name. Olfactomedin domain-containing proteins are of ten found in neural tissues. Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum. In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation. The human Olfactomedin-2 cDNA encodes a 20aa signal sequence and a 454 mature protein that contains two coiled-coil regions (aa58-85 and 136-193) and an Olfactomedin domain (aa194-446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus , and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2. Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients. The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noel in-1 might heterodimerize with Olfactomedin-2. Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication.
Source:Chinese Hamster Ovary cell line, CHO-derived. Gln21-Pro454, with a C-terminal 6-His tag.
Biological Activity:Measured by the ability of the immobilized protein to support the adhesion of HEK293T human embryonic kidney cells. When 5x10er cells/well are added to human Olfactomedin-2 coated plates (1ug/ml, 100ul/well), ~50-75% will adhere after 1h at 37°C.
Endotoxin: ≤1EU/ug (LAL)
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with 100ug/ml PBS. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, Chinese Hamster Ovary cell line
Grade：Purified
Purity：≥90% (SDS-PAGE)
Form：Supplied as a lyophilized powder from PBS. Carrier free. Reconstitute with 100ug/ml PBS.
