Integrin a10b1 is one of twelve integrin family adhesion receptors that share the b1 subunit. The non-covalent heterodimer of 160kD a11 and 130kD b1/CD29 type I transmembrane glycoprotein subunits is expressed mainly on chondrocytes within cartilage, but also infibrous connective tissues such as heart valves and ligaments. The a10 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site. The b1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail . The dimer is folded when it is least active. Divalent cations and intracellular (inside-out ) signaling convert it to its most active, extended and open conformation. The 1100aa human a10 extracellular domain (ECD) shares 88-92% aa sequence identity with mouse, rat , canine and bovine a10, while the 708aa human b1 ECD shares 92-96% aa sequence identity with rat , bovine, mouse, and feline b1. A reported a10 splice variant lacking ECD aa974-1012 was not expressed on the cell surface. I domain-containing b1 integrins a1b1, a2b2, a10b1 and a11b1 all bind collagens; all but a11b1 also bind laminins. During cartilage differentiation, a10b1 is thought to be the main integrin binding type II and IX cartilage collagens. However, deletion of mouse a10 causes a mild phenotype including slightly shortened bones and narrowed hypertrophic zones, indicating that another collagen-binding integrin, likely a2b1, may compensate for a10b1 functions. Migration of melanoma cells has been noted to correlate with a10b1 expression.
Recombinant non-covalent heterodimer corresponding to Phe23-Ser1122 of human Integrin a10, acidic tail at N-terminal and HHHHHH at C-terminal Accession # (O75578) and Gln21-Asp728 of human Integrin B1, basic tail at N-terminal Accession # (P05556), expressed in CHO cells
Biological Activity:Measured by its binding ability in a functional ELISA. When mouse Laminin is coated at 10ug/ml, Integrin a10b1 binds with an apparant Kd<5nM.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, Chinese Hamster Ovary cell line
