(IRAKs) are important mediators in the signal transduction of the Toll/IL-1 receptor (TIR) family. Members of the TIL family play a role in innate immune responses by coordinating host defense mechanisms. IRAK4 is the most recently discovered IRAK protein. IRAK-4 has been shown to interact with IRAK-1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK-4 has been shown to activate NFkB as well as mitogen-activated protein (MAP) kinase pathways. Studies demonstrate that mice lacking IRAK4 are severely impaired in their IL-1, Toll-like receptor (TLR) signaling and response to viral and bacterial challenges; this indicates that IRAK4 has an important role in innate immunity. Humans lacking IRAK 4 exhibit pyogenic bacterial infections, while their blood and fibroblast cells fail to activate NF-kB. IRAK-4 is believed to be required for the LPS-induced activation of antigenpresenting cells.
Source:Recombinant human IRAK4, aa2-end, with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system.
Applications:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:The enzyme reaction was carried out for 1h at room temperature in reaction buffer. Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
