GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.
Source:Recombinant Aequorea victoria GFP, full length, with C-terminal HIS tag, expressed in E. coli .
Molecular Weight: 28.6kD
Absorbance:395 and 470nm, emits green fluorescence at 509nm.
Applications:Suitable for use as a quantitative control for GFP expression studies using GFP reporter vectors. It can also be used as a standardon protein gels and western immunoblots or for calibration of Fluorometers, flow cytometers and FACS machines. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
仕様
Size：200ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：≥90%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 50% glycerol.
