The L858R mutation has been associated with non-small cell lung cancer patients who demonstrate clinical responsiveness to the tyrosine kinase inhibitor gefitinib (Iressa, ZD1839).
Source:Recombinant human EGFR T790M L858R, aa668-1210, with N-terminal GST tag, expressed in baculovirus expression system.
Molecular Weight:~88kD
Specific Activity:As reported
Reaction Buffer:50mM HEPES (pH7.5), 10mM MgCl2, 1mM EDTA, 0.01% BRIJ- 35 and 200uM of ATP.
Substrate:4uM Tyr peptide 4 from Invitrogen.
Applications:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:The enzyme reaction was carried out for 1h at room temperature in reaction buffer. Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
仕様
Size：10ug
Source Antigen：Recombinant, Baculovirus
Grade：Purified
Purity：≥80%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 138mM NaCl, 0.05% Tween 20, 50% glycerol, 3mM DTT.
