Recombinant Human FGF-acidic (FGF-1) is a single, non-glycosylated, polypeptide chain containing 15aa and having a molecular weight of 17.4kD. The sequence of the first five N-terminal amino acids was determined to be Met-Phe-Asn-Leu-Pro. Contains less than 1% dimers and aggregates.
Source: Recombinant Human FGF-acidic, expressed in E. coli.
Molecular weight: 17.4kD
Endotoxin: ≤0.1ng/ug (IEU/ug).
Activity: The ED50 as determined by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10ng/ml, corresponding to a specific activity of 1x10e5Units/mg.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Stable for at least 6 months at -20°C. Reconstitute with sterile deionized water to a concentration ≥100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (HPLC, FPLC, SDS-PAGE).
Form：Supplied as a lyophilized powder in 10mM Tris, pH 7.6, 100mM sodium chloride. No preservative added. Reconstitute with sterile deionized water to a concentration ≥100ug/ml.
