Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The Streptavidin is isolated by purification from fermentation broth. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react unspecifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.
Source:Streptomyces avidinii
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2ml
Source Antigen：Streptomyces avidinii
Grade：Purified
Purity：Purified. Calf intestinal alkaline phosphatase conjugated to Streptavidin (Streptomyces avidinii).
Form：Supplied as a liquid in conjugate stabilizing buffer, 0.01% methylisothiazolone, 0.01% bromonitrodioxane. Labeled with AP.
