Contains Nucleocapsid protein immunodominant region (aa1-49/192-220). Contains GST fusion partner. Immunoreactive with SARS positive sera.
Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). SARS is caused by a human coronavirus, which are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31kb). The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46kD nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. The nucleocapsid protein of SARS shares little homology with nucleocapsid proteins of other members of the coronavirus family. Nucleocapsid proteins of other coronavirus have been reported to be involved in forming the viral core and also in the packaging and transcription of the viral RNA.
Source: E. coli
Molecular Weight:~35kD. May appear as multiple bands on SDS-PAGE (intact protein at 35kD, GST fusion protein at 26kD and the Nucleocapsid fragment at 9kD).
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~95% pure (10% PAGE Coomassie staining). GS-4B Sepharose Affinity Purification.
Form：Supplied as a liquid in 50mM Tris-HCl, 60mM sodium chloride, 50% glycerol.
