Contains a mosaic of the immunodominant regions of the C-terminal of the Spike protein. Not full-length spike protein. Contains GST fusion partner. Immunoreactive with SARS positive sera.
A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139kD protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells.
Source: E. coli
Molecular Weight:~37kD. May appear as multiple bands on SDS-PAGE (intact protein at 37kD, GST fusion protein at 26kD, and the Spike fragment at 11kD).
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：≥95% pure (10% PAGE Coomassie staining). GS-4B Sepharose Affinity Purification.
Form：Supplied as a liquid in 25mM Tris-HCl, 0.4% sarcosyl, 0.25% Triton-X100, 50% glycerol.
