Recombinant Human Leptin is a single non-glycosylated polypeptide chain containing 146aa and having a molecular weight of 16kD. The sequence of the first five N-terminal amino acids was determined to be Ala-Val-Pro-Ile-Gln.
Source: E. coli
Molecular Weight:~16kD
Biological Activity:ED50 calculated by the Leptin-dependent stimulation of Human OB-R transfected mouse BaF3 indicator cells is 0.5-1.6ng/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (RP-HPLC and SDS-PAGE).
Form：Supplied as a lyophilized powder from 0.0045mM sodium bicarbonate. No preservative added. Reconstitute with ddH2O.
