Endothelin-converting enzymes (ECEs) hydrolyze a specific peptide bond of big endothelins to produce active endothelins, some of the most potent vasoconstrictors known. ECE-1 is a member of the M13 zinc metallopeptidase family. Other members of the M13 family include thermolysin, neprilysin, Kell , and ECE-2. M13 metallopeptidases can be distinguished from other metallopeptidases by their sensitivity to inhibition by phosphoramidon. ECE-1 is most highly expressed in the cardiovascular endothelium, but is also expressed in some endocrine tissues. ECE-1 is known to hydrolyze a variety of bioactive pepti des, including bradykinin, neurotensin, angiotensins, and Substance P, with a substrate specificity similar to that of neprilysin. ECE-1 displays pronounced pH dependence in its substrate specificity. The degradation of Substance P by ECE-1 in endosomes regulates beta-arrestin-dependent ERK-2 signaling to prevent cell death in some neuronal cells. Four isoforms of ECE-1 are present in humans and mice, all of which encode a Type II integral membrane protein. The four isoforms share a common extracellular catalytic domain, differing in their N-terminal cytoplasmic tail regions. The recombinant mouse ECE-1 transmembrane and cytoplasmic tail domains were replaced with a signal sequence, resulting in the secretion of the soluble catalytic ectodomain.
Recombinant protein corresponding to Gln89-Trp769 of mouse ECE-1 with a N-terminal 6-His tag, expressed in CHO cells. UniProt Accession: Q4PZA2.
SDS-PAGE:~90-130kD, reducing conditions.
Biological Activity:Measured by its ability to to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is > 2250pmoles/min/ug
Storage and Stability:For long-term storage, aliquot and store at -70°C. Aliquots are stable for 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Chinese Hamster Ovary (CHO) cell line
Grade：Purified
Purity：≥90% (SDS-PAGE) under reducing conditions and visualized by silver stain Endotoxin: ≤1EU/ug (LAL)
Form：Supplied as a liquid in 25mM Tris base, 150mM sodium chloride, pH 7.5, 5mM ZnCl2
