Galectin-3 binding protein (Galectin-3BP), called CyCAP (cyclophilin C-associated protein) in the mouse, is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) super family. Mouse Galectin-3BP is generally considered the paralog of human Galectin-3BP, which is also called MAC-2 binding protein (MAC-2BP), or 90kD tumor associated antigen (TAA90K or 90K). However, mouse Galectin-3BP binds cyclophilin C but not Galectin-3, while the opposite is true of human Galectin-3BP. The 577 amino acid, 77-95kD mouse Galectin-3BP contains an 18aa signal sequence and four definitive domains. Domain 1 is a group A scavenger receptor domain, domain 2 is a BTB/POZ domain that mediates dimerization, and domain 3 is an IVR domain that is also found following the POZ domain in Drosophila kelch protein. Little is known about domain 4. Mature mouse Galectin-3BP shares 88%, 69%, 67% and 65% aa identity with rat, human, dog and cow Galectin-3BP, respectively. The Galectin-3BP dimer interface residues are generally conserved between mouse and human, so linear dimers that occur in humans are also likely in the mouse. Human Galectin-3BP also forms ring-shaped decamers and dodecamers. Mouse Galectin-3BP is widely expressed and modifies innate immune reactions. It is stimulated in macrophages by adhesion, TNF-a or IFN-g. Mice deficient in Galectin-3BP secrete increased amounts of IL-12, TNF-a and IFN-g and have lower survival in response to endotoxin. Galectin-3BP is induced in rat skin fibroblasts, keratinocytes, macrophages and lymphocytes during wound healing, and wounded Galectin-3BP-deficient mice are slow to heal. Rat Galectin-3BP is upregulated after cerebral ischemia. Ligands for human Galectin-3BP include some other galectins, collagens and integrins, but remain to be tested for mouse Galectin-3BP binding.
Recombinant protein corresponding to Thr19-Val577 of mouse Galectin-3 Binding Protein, with C-terminal 6x His tag expressed in NSO cells. Accession Number: NP_035280
SDS-PAGE:80-90kD, reducing conditions
Biological Activity:Measured by its binding ability in a functional ELISA. When rmMAC-2BP is coated at 1ug/ml (100ul/well), rhGalectin-3 binds with an apparent KD <5nM.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size：50ug
Source Antigen：Recombinant, NS0 cells
Grade：Purified
Purity：≥90% (SDS-PAGE under reducing conditions and visualized by silver stain). Endotoxin: ≤0.1EU/ug (LAL)
Form：Supplied as a lyophilized powder from 20mM MOPS, 100mM sodium chloride, 10mM Imidazole, 5% trehalose. Reconstitute with 167ul of sterile PBS.
