Keratins are a class of fibrous proteins or scleroproteins important both as structural proteins and as keys to the study of protein conformation. The family represents the principal constituent of epidermis, hair, nails, horny tissues, and the organic matrix of tooth enamel. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms an alpha-helix, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.
Recombinant Human Cytokeratin 14 is a single,non-glycosylated polypeptide chain having a molecular mass of 51,530 Dalton.
Dimers and Aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of cytokeratin 14 as a Reference Standard.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: ≤ 0.1ng/ug (IEU/ug).
