Hemoglobin is composed of one globin plus four hemes; heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520; however, ultracentrifugal and osmotic pressuremeasurements indicate an actual molecular weight four times the minimum molecular weight.
Solubility (10mg/ml, H2O):Dark red brown
Iron (Fe):0.25-0.35%
Water (Karl Fischer):≤8%
Storage and Stability:Lyophilized powder may be stored at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Note: Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin. Opened bottles should be capped tightly and kept in a dark, low humidity environment.
Country of Origin: USA
Source: Porcine bloodForm: Dark red/brown
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
