The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also known as high endothelial cells Nacetylglucosamine 6Osulfotransferase (HECGlcNAc6ST) or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O glycans, but also has activity toward core 3 type of O glycan 5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.
Source:Chinese Hamster Ovary cell line (CHO) derived recombinant protein corresponding to His29-His386 from human Carbohydrate Sulfotransferase 4, fused with an N-terminal 6 His tag, Accession# NP_005760.
N-terminal Sequence Analysis:His
Predicted Molecular Mass:43kD
SDS-PAGE:45kD, reducing conditions
Specific Activity:>90pmoles/min/ug
Activity:Measured by its ability to transfer sulfate from PAPS to disaccharide acceptor B-D-GlcNAc-(1->6)-a-D-Man-1->OMe.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Source Antigen：Recombinant, CHO cells
Grade：Purified
Purity：≥85% (SDS-PAGE under reducing conditions and visualized by silver stain). Endotoxin: ≤1EU/ug (LAL)
Form：Supplied as a liquid in 20mM Tris, pH 7.5, 120mM sodium chloride, 20% glycerol.
