Interleukin-4 (IL-4) is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naïve helper T-cell to Th2 cells. Interleukin-4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.
Endotoxin Level:≤1.0 EU per 1 ug of protein (determined by LAL method)
Biological activity:The ED50 for this effect is <1.8ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥ 95% by SDS-PAGE
Form：Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.
