FGF-21 is a unique member of FGF super family that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways.
Recombinant protein corresponding to aa29-209 frin human FGF-21, fused to His-Tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Biological Activity:The ED50 for this effect is < 12 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell (which corresponds to > 83,000 units/ml).
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：≥90% (SDS-PAGE) Endotoxin: ≤1EU/1ug (LAL)
Form：Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.
