Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family which belongs to the TGF-β superfamily. This protein is a vital regulatory molecule that functions throughout bone and cartilage development, specifically tooth development, limb formation, bone induction, and fracture repair. In human embryonic development, BMP- 4 is required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. And it is overexpressed in patients with fibrodysplasia ossificans progressiva. Recombinant BMP-4 was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Endotoxin Level:≤ 1.0 EU per 1 ug of protein (determined by LAL method)15% SDS-PAGE (3ug)
Storage and Stability:For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥ 95% by SDS-PAGE
Form：Supplied as a liquid in 10mM sodium citrate buffer, pH 3.5, 10% glycerol.
