Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho- transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis.
Source:Recombinant Human AK2, fused to his-tagged, expressed in E. coli, (1-239aa).
Enzyme Activity:Specific activity is > 25 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37°C.
Endotoxin Level: ≤1.0 EU/1ug (LAL).
Storage and Stability:For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Purified: ≥95% by SDS-PAGE.
Form：Supplied as a liquid in 20mm tris, pH7.5, 5mm DTT, 20% glycerol.
