Three types of interferons have been described: interferon-alpha (IFN-alpha), interferon-beta (IFN-beta), and interferon-gamma (IFN-gamma). An extensive variety of biological effects have been observed for the interferon family including antiviral activity; anti-proliferative activity; stimulation of cytotoxic activities in lymphocytes, natural killer cells, and macrophages; and modulation of the major histocompatibility complex. Only one mouse-IFN-beta gene has yet been described. It codes for a 161 amino acid polypeptide (20kD). Mouse-IFN-beta binds to the type I interferon receptor. It is not recognized by antibodies directed against mouse alpha interferon or mouse gamma interferon.
Source:CHO cells
Recommended Dilution:For dilutions use a buffer containing 2% bovine serum albumin or culture medium. (Do not use fresh serum.)
Unit Definition:One unit of mouse beta interferon is the amount of beta interferon which protects 50% of the indicator cell population from viral destruction. One unit of Mouse Beta Interferon approximates the bioactivity contained in one unit of the mouse beta interferon standard prepared by NIH (Gb02-902-511).
Application Notes:Activity is an average of mulitple determinations. Be aware that activity is dependent on the type of IFN assay and vary between labs. Bioactivity was determined using IFN-β sensor cells by monitoring the activation of the JAK/STAT/ISGF3 pathway.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Once reconstituted, store stock solution in aliquots at -20°C. Reconstituted product is stable for 6 months at -20°C. Note: Mouse IFN-beta is highly labile when agitated. Repeated freeze and thaw cycles and prolonged exposure to light will cause loss of activity.
仕様
Size：50KU
Source Antigen：Recombinant, CHO cells
Grade：Purified
Purity：Purified
Form：Supplied as a powder lyophilized from a solution containing PBS, 10% mouse albumin and 0.01% gelatin. Reconstitute the vial by injection of 1ml distilled or de-ionized water (Caution: vial is under vacuum).
