The immunoregulatory cytokine tumor necrosis factor alpha (TNFa) recognizes two cell surface receptors. TNF-R1 (also known as CD120a) has an apparent molecular mass of 55/60kD and is constitutively expressed on all nucleated cells, whereas TNF R2 (also known as CD120b) with an apparent molecular mass of 75/80kD, is restricted to hematopoietic cells. TNF-R2 directs TNFa mediated activation and proliferation of T lymphocytes. An autocrine role for TNF and TNF-R2 is implicated in hematopoietic malignancies, and the TNF-R2 locus is a translocation target in several leukemias, suggesting a role for TNF-R2 in the development of certain leukemias. The p55 receptor extracellular domain was produced in insect cells (Sf9) infected with recombinant viruses.
Source:Sf9
Biological Activity:Binding of the ligand TNF was demonstrated by receptor binding competition of binding with radioactive TNF.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9
Grade：Purified
Purity：Purified. ≥ 80% pure. Three bands are observed by SDS gel electrophoresis.
Form：Supplied as a lyophilized powder in PBS, 10mM Hepes, pH 7.0
