Human PDGF-AA homodimer is produced by genetically engineered yeast, and purified by ionic exchange chromatography and reverse-phase HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds.
Biological Activity:Human PDGF-AA homodimer is fully active as measured by the mitogenic assay involving the stimulation of 3H-thymidine incorporation into NIH-3T3 cells.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Yeast
Grade：Highly Purified
Purity：≥ 95% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis.
