Human M-CSF is produced in genetically engineered E. coli cells, and purified by sequential chromatography, refolding and HPLC. This polypeptide is a disulfide-linked dimeric protein (1).
Biological Activity:The biological activity of human M-CSF is measured using the mouse bone marrow colony-forming assay (2). The specific activity is 6 X 10e7 U/mg.
Storage and Stability:Store at -20°C. Product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Caution: Do not use at pH lower than 5; product is less stable under this condition.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：Purified. ≥ 92% pure by aa composition and SDS gel electrophoresis.
Form：Supplied as a liquid containing 0.1M citrate (pH 6.5) and 0.1% mannitol.
