Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera.
All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then constructed into one recombinant antigen.
The protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions.
Immunoreactivity:Immunoreactive with sera of HCV-infected individuals, including sera with antibodies to NS3 secondary structure domains.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：E. coli
Grade：Affinity Purified
Purity：Purified by affinity chromatography. ≥95% by 10% PAGE (coomassie staining)
Form：Supplied as a liquid 50mM sodium phophate, pH 8.5, 2.4mM EDTA, 5mM DTT, 0.1% SDS.
