85-1545-73　MGMT, Recombinant, Human (O6-Methylguanine DNA Methyltransferase) 100ug　M3884-91C

特徴

• O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques.
• Source:Recombinant corresponding to aa1-207 of human MGMT (His-tagged) expressed in E.coli.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYFHQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSGGLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
• Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
• Applications:Suitable for use in Western Blot. Other applications not tested.
• Recommended Dilution:Western Blot: 3ug/ml 15% SDS-PAGE, ~23.8 kD
• Optimal dilutions to be determined by the researcher.
• Endotoxin: ≤1.0 EU/ug of protein (LAL)
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.