85-1545-30　Single-Stranded DNA Binding Protein, E. coli (SSB) 50ug　S1013-86R

特徴

• E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kD subunits. It binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA. It is involved in DNA replication and in recombination in vivo.
• Spectral Ratio A280/A260:As Reported
• Binding Activity:~5ug of SSB protein is required to prevent optical density change of 1ug of single-stranded DNA upon addition of 10mM MgCl2.
• Isoelectric Point:6.0
• Nucleotides Bound Per Monomer:8-16
• Thermostability:SSB retains activity after 20 min incubation at 65°C.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

仕様

• Size：50ug
• Source Antigen：E. coli strain containing an overproducing clone of E. coli SSB protein.
• Purity：≥95% (SDS-PAGE)
• Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 200mM sodium chloride, 0.1mM EDTA, 1mM DTT, 50% glycerol
• EU Commodity Code：38220090
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