Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. The tissues were excised, immediately frozen by liquid nitrogen and then stored in -80ºC. All the tissue are pathologically identified. A single tissue section with 5-10um thickness is mounted on a positively charged glass slide. The slide is fixed and dehydrated with cold acetone for consistent results with in situ hybridization and immunohistochemistry.
Frozen tissue section line encompasses a large selection of tissue sections which are good for immunohistochemistry and in situ hybridization. It includes human adult and fetal normal tissues, human diseased and tumor tissues, as well as from mouse, rat, and monkey tissues. The tissue sections are approximately 5-10um thick. All the tissues are diagnosed by a licensed pathologist. Accurate documentation and clinical histories of the tissues can be supplied upon request.
Features:
Tissues from a wide variety of hard to obtain tissue sources
Suitable for both immunohistochemistry and in situ hybridization
Multiple tissues or a single tissue per slide
Documentation on tissues' clinical histories available
Applications:
Cellular localization of tissue specific mRNA and protein expression
Identification of tumor and disease specific genes, proteins by in situ hybridization, immunohistochemistry, and in situ PCR detection
Comparison of the locations of novel genes in different tissues
Control slides for immunohistochemistry studies
Preparation:Tissues were snap frozen in liquid nitrogen immediately after excision, and embedded in OCT. All frozen tissue products, including frozen tissue arrays, frozen tissue sections, and frozentissue panels, can be used for both immunohistochemistry and in situ hybridization. These products are ideal for rapid cellular localization of RNA and protein expression, especially for antibodies that don't recognize paraffin embedded tissue. At least one of the tissue slides from each lot was stained with H & E to ensure the quality. The tissue sections are 5-10um in thickness, mounted on positively charged glass slides, and fixed by cold acetone.
Storage and Stability:Store fresh frozen tissue section slides -70ºC. Stable for 6 months from the date of receipt under proper storage conditions.
