Recombinant full length Creatine Kinase MM isoenzyme without the C-terminal lysine on both subunits. CK-MM is a 47kD dimeric protein comprised of 2 identical subunits of Type 1 sequence. Purified in the enzymatically active form. Reacts with polyclonal antibodies specific to MM isoenzyme in ELISA.
Applications:Suitable for use in ELISA and Western Blot. Major band migrating above 37kD in Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Unit Definition:One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37°C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction.
Source: Pichia pastoris
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
仕様
Size：1mg
Source Antigen：Recombinant, Pichia pastoris
Grade：Highly Purified
Purity：~95% (SDS-PAGE). Purified under non-denaturing conditions.
Form：Supplied as a liquid in 0.01M Tris-HCl, pH 7.0-7.5, 0.075M sodium chloride, 10mM beta-mercaptoethanol, 0.09% sodium azide, 50% glycerol.
