Recombinant full length Creatine Kinase MB Isoenzyme with aa sequence identical to native enzyme. CK-MB is a dimeric protein comprised of M and B subunits. The doublet band migrating approximately 47kD is not resolved on most gels. Purified in the enzymatically active form. Reacts with monoclonal antibodies specific to MB isoenzyme in ELISA.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Unit Definition:One unit equals 1 micromole creatine phosphate formed/minute.
Enzyme Activity:≥700u/mg at 37°C at 340nm
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Source Antigen：Recombinant, Pichia pastoris
Grade：Purified
Form：Supplied as a liquid in 10mM Tris-HCl, pH 6.8, 0.5mM DTT, 0.5mM EDTA, 50% glycerol. No preservative added.
