85-1544-22　Purification Media, BioSeparation™ Affinity, Protein G (Agarose) 1ml　P9100-85

特徴

• Protein G originated from Streptococci is a monomeric protein with a molecular weight of about 50,000kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of immunoglobulins from mouse, rabbit, goat and human. Due to this binding ability, Protein G is commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).
• Matrix:SepharoseTM CL-4B
• Coupling Method: TargetLock
• Protein G Density: 3-4mg/ml of gel
• Binding Capacity:~4-8mg rabbit IgG per ml of beads
• Mean Bead Size:40-165um
• Bead Structure:Highly cross-linked spherical agarose, 4%
• Max Back Pressure:0.3MPa, 3 bar
• Max. Flow Rates: 4ml/min/cm2
• Recommended Flow Rate: 1-3 ml/min/cm2
• Stability of the Matrix:pH 2-11.
• Storage:Store gel refrigerated at 4°C in PBS pH 7.4 containing 0.1% sodium azide as a preservative, or PBS pH 7.4 containing 20% ethanol.