85-1544-18　［取扱停止］Purification Media, BioSeparation™ Affinity, Protein A Beads (Agarose) 5ml　P9100-75

特徴

• The origin of Protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from goat, rabbit, mouse and human
• Protein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to agrose beads using TargetLock chemistry, in order to create a more stable product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins.
• Protein A beads are being routinely used for the isolation of IgG from several species of mammals. The binding of Protein A to IgGs vary between animal species and between IgG subclasses within the same species. Major limitation of protein A lies with the weak binding itpresents towards mouse IgG1, a common IgG subclass. Despite that, Protein A possesses usefulproperties that make it a popular choice for the isolation of most types of IgGs. Protein A binds IgGs through the Fc region of the molecules leaving the Fab region available for binding the antigen. Protein A Beads are commomnly used for antibodies purification and for the isolation of immune complexes by Immunoprecipitation.
• Protein A Beads Specifications:Matrix:Sepharose CL-4B
• Coupling method: TargetLock.
• Protein A Density:2-4mg/ml of resin
• Binding Capacity:~4-8mg rabbit IgG per ml of resin
• Mean bead size:40 -165um
• Bead structure:Highly cross-linked spherical agarose, 4%
• Max back pressure: 0.3 MPa, 3 bar
• Max. flow rate:4 ml/min/cm2
• Recommended flow rate:1-3 ml/min/cm2
• Stability of the matrix:pH 2-11
• Storage and Stability:May be stored at 4°C. Do not freeze. Stable for at least 6 months.