85-1544-16　［取扱停止］Purification Media, BioSeparation™ Affinity, Protein A, Recombinant 50mg　P9100-70

特徴

• Recombinant Protein-A used also in Protein A beads (P9100-75) is a genetically engineered form of Protein-A derived from Staphylococcus aureus. Non-essential regions have been modified while leaving the IgG binding sites intact. Protein A is been used extensively used for the isolation of IgG from several species of mammals. The affinity of Protein A to IgG's is different between species and IgG subclasses within a given species. A major known limitation of protein A is its weak binding to Mouse IgG1– a major immunoglobulin subclass in Mice. Despite its variable binding characteristics, Protein A is a popular choice for both antibodies binding in various assays and when coupled to beads, for Immunoprecipitation of -antigen complexes. Protein A binds specifically the Fc region of immunoglobulins leaving the Fab region free for binding the antigen. Hence, Protein A is extremely useful for isolating of immune complexes.
• Protein A Beads characteristics:Source:Recombinant protein
• Molecular Weight:23.3kD expressed in E. coli
• Purity:≥ 90% by SDS-PAGE
• Spectroscopic analysis:OD280nm/250nm=2.51
• Endotoxin:≤ 0.5EU/mg Recombinant Protein A (LAL)
• Composition:Lyophilized, white powder. 10.9mg Recombinant Protein A, 0.82 mgphosphate buffer salts.
• Storage before Reconstitution: Store at 4°C. Product is stable under these conditions forone year.
• Storage after Reconstitution: Store at 4°C in PBS pH 7.4 added with sodium azide 0.1% (w/v) as a preservative. Product is stable under these conditions for 1 month. Alternatively, prepare small aliquots and store at -20°C for up to two years. Avoid repeated freezing and thawing cycles.
• Storage and Stability:Stable at RT for 1 week