Principle of the Test:Anti-Nuclear Antibodies (ANA) ELISA kit is based on binding of mouse ANA IgG+IgA+IgM from serum samples to extracted nuclear antigen immobilized on microtiter wells. After a washing step, IgG (HRP) goat anti-mouse is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (blue color) is directly proportional to the amount of ANA present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. The activity of total mouse antibodies in samples is calculated relative to mouse ANA calibrators.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
