The Protein Expression Rescue Kit is intended to help maximize the amount of soluble protein produced during expression of recombinant proteins in E. coli. This comprehensive kit includes samples of Expression Media and two reference media, Glucose M9Y and LB (Miller) Broth. The Expression Media are specially designed to increase the production of recombinant proteins. The kit also includes new medium additives: LB Booster and Augmedium. LB Booster is designed to increase the bio-mass production of any expression medium up to 5-fold. Augmedium is a medium pre-conditioner that induces the expression of chaperone proteins in advance of expression of the target recombinant protein. This can increase the amount of soluble protein accumulated.
Application:Maximizes the amount of soluble protein produced during expression of recombinant proteins in E. coli.
Reagent Preparation:Dissolve the contents of the LB Booster packet in 20mL deionized water and filter sterilize using a 0.2um filter. The stock solution will be 20X. Store at 4°C. Dissolve the contents of the Augmedium container in 25mL deionized water and fi lter sterilize using a 0.2um filter. Aliquot 5mL portions into sterile 15mL conical tubes and store at -20°C. The stock will be a 50x solution. For use, snap thaw at 37°C and store at 4°C for no more than five (5) days.
Storage:Store powdered media at RT, except store P9102-41B8 powder at -20°C. Prepared media should be kept at 4°C and used within a short period of time.
pEX™ is a trademark of United States BiologicalProtein Expression Rescue Kit™ is a trademark of Athena Enzyme Systems™
