Human, recombinant, full length with C-terminal His tag expressed in Sf9 cells.
Applications:Suitable for use in enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Specific Activity:~400pmol/min/ug
Recommended Dilution:Assay Conditions:50mM Tris-HCl, pH 8.0, 137mM sodium chloride, 2.7mM of KCl, 1mM MgCl2, 20uM substrate, 5ug BSA, H1827-53B. Incubate at 37ºC for 30 minutes
Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
仕様
Size:50ug
Source Antigen:Recombinant, Sf9 cells
Grade:Affinity Purified
Purity:~ 70% by SDS-PAGE
Form:Supplied as a liquid in 40mM Tris, pH 8.0, 100mM sodium chloride, 2.2mM KCl, 0.04% Tween 20, 20% glycerol.