85-1542-85　［取扱停止］Elk-1, aa307-428, Recombinant, Human 10ug　E2240-26

特徴

• Elk-1 is a transcription factor that is activated by mitogen-activated protein kinases (MAPKs) and whose main function is the regulation of growth-related proteins in response to extracellular stimuli. Because of the importance of Elk-1 to the regulation of successful cell growth, accurate monitoring of Elk-1 in cells, tissues and animals is crucial for many biomedical research and drug development projects.
• Description:Recombinant Human ELK-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing 122 amino acids (307-428) and having a molecular mass of 60kD. This protein is conjugated with N-terminal His-tag and C-terminal myc tag (EQKLISEEDL). Recombinant Elk-1 is purified by proprietary chromatographic techniques.
• Biological Activity:Recombinant human Elk-1 is phosphorylatable in vitro, using either recombinant active ERK1/2, or with immunoprecipitated ERK1/2 from stimulated cells. This phosphorylation can be monitored by Western blot analysis using BioSource's phosphorylation site specific antibody directed to Elk-1 [pS383] in conjunction with chemiluminescence detection methods. Please note: Kinase activity may vary depending on the substrate and reaction conditions.
• Storage:Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.