Human BTK (GenBank Accession No. NM_000061), full length with N-terminal GST tag, MW= 106kD, expressed in an Baculovirus infected Sf9 cell expression system.
Application:Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications have not been tested.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Specific Activity:~20 pmol/min/ug
Assay Conditions:Enzyme reaction was carried out for 1 hour at RT in a buffer containing 50mM HEPES, pH 7.5, 10mM MgCl2, 1mM EDTA, 0.01% BRIJ-35, 200uM ATPSubstrate: 2uM Tyr peptide 1.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
