85-1542-76　Phosphoinositide 3 Kinase, Recombinant, Human, p120 gamma, full length, N-terminal His-Tag, (PI3K) 20ug　P4073-26F

特徴

• Human PI3K (p120 gamma) (GenBank Accession No. AF327656), full length with N-terminal His tag, expressed in an Baculovirus infected Sf9 cell expression system (MW:~131kD).
• Specific Activity: Lot-dependent. Sample Value: ~1200 pmol/min/ug
• A 50ul kinase reaction is conducted in a buffer containing 40mM Tris-HCl (pH 7.5), 20mM MgCl2, 100uM ATP, 200uM L-alpha-phosphatidylinositol/ L-alpha-phosphatidylserine (PI/PS), and 20-200ng enzyme for 15 min at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (PromegaCorporatio).
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage and Stability:Store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

仕様

• Size：20ug
• Source Antigen：Recombinant, Baculovirus infected Sf9 cells
• Grade：Purified
• Purity：≥80% by SDS-PAGE
• Form：Supplied as a liquid in 25mM Tris, pH 8.0, 69mM NaCl, 1.35mM KCl, 0.025% Tween 20, 3mM DTT, and 50% glycerol
• Molecular Weight Non Ab：131
• Swiss Prot Number：P48736
• EU Commodity Code：30021019
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