Human Sphingosine kinase 1 or SphK1 (GenBank Accession No. AF200328), full length with N-terminal His-tag, MW=46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D- erythro-sphingosine and [ -32P]ATP as substrates at pH 7.4 and 30 °C. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min.
Assay buffer: 50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5 mM ATP, uM D-erythro-sphingosine, and 0.5ug/ml sphingosine kinase 1.
Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, human from Baculovirus infected Sf9 cells
Grade：Purified
Purity：≥80% by SDS-PAGE
Form：Supplied as a liquid in 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.
