85-1542-65　Plk3, Recombinant, Human, N-terminal His-Tag, Serine Threonine Kinase, aa 58-339 (CNK, FNK, PRK, Polo-like kinase 3) 10ug　P4275-07B

特徴

• Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal HIS6 tag,
• MW 36.7kD, expressed in an Baculovirus infected Sf9 cell expression system.
• Purity: >80% by SDS-PAGE.
• Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of casein substrate per minute at pH 7.4 and 30°C
• Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 2mg/ml casein, 0.1mM ATP and 0.1ug/ml Plk3.
• Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months

仕様

• Size：10ug
• Source Antigen：Recombinant, human from Baculovirus infected Sf9 cells
• Purity：≥ 90% by SDS-PAGE
• Form：25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.
• Calc Crossreactivity：Hu
• Molecular Weight Non Ab：36.7
• EU Commodity Code：30021019
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