Human transmembrane leukocyte antigen related PTP (LAR), GenBank accession No. NM_130440, a.a. 1330-1620 (catalytic domain) with N-terminal GST-tag, MW=58 kDa, expressed in an E. coli expression system.
Specific Activity: 1 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 °C.
Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP.
Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Source Antigen：Recombinant, Human from E. coli
Grade：Highly Purified
Purity：>95% by SDS-PAGE.
Form：Supplied as a liquid in 25 mM Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 2mM EDTA, 1 mM DTT, 10 mM glutathione.
