Human Aurora kinase B (GenBank Accession No. NM_004217), (a.a. 2-344), expressed in an Baculovirus infected Sf9 cell expression system.
Stability: 6 months at –80 °C
Purity: >95% by SDS-PAGE.
Specific Activity: 22 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30 °C.
Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 0.2 ug Aurora B kinase.
Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
仕様
Size：10ug
Source Antigen：Recombinant, human from Baculovirus infected Sf9 cells
Form：25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 3mM DTT.
