Vimentin is a 57kD class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin. The human Vimentin cDNA encodes a 466aa protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers. Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate filaments. These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress. IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons. Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge. Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis. Phosphorylation promotes secretion of Vimentin by TNF-alpha-stimulated macrophages. Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst. Cellassociated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes.
Recombinant protein corresponding to aa2-466 from human Vimentin with a six-histidine tag at the C-terminus, expressed in E. coli (P08670).
Molecular Weight:Calculated molecular mass of ~ 54.4kD. Based on N-terminal amino acid sequencing, the recombinant protein begins with Ser 2.
Activity:Measured by the ability to bind recombinant human NKp46/NCR1 Fc Chimera in a functional ELISA with an estimated KD <2nM.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 4mM HCL. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E .coli
Grade：Highly Purified
Purity：≥95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤1EU/ug (LAL).
Form：Supplied as a lyophilized powder in 10% acetonitrile, 0.1% TFA. Reconstitute with sterile 4mM HCl to a concentration of 100ug/ml.
