Mouse THSD1 (Thrombospondin type-1 domain-containing protein 1), also known as Transmembrane molecule with thrombospondin module (Tmtsp), is a 95kD, type I transmembrane protein (1). It is synthesized as a precursor that is 851 amino acids (aa) in length, and has a 24 aa signal sequence, a 388 aa extracellular region, a 21 aa transmembrane region, and a 418 aa cytoplasmic region. The extracellular region contains six potential N-linked glycosylation sites and the thrombospondin type-1 (TSP1) domain, which is implicated in cell adhesion and migration (1). Also present in the molecule are 9 PKC-phosphorylation sites, 3 Ig-like domains, and a tyrosine-phosphorylation site by its C-terminus (1). Mouse THSD1 shares 78% aa sequence identity with human THSD1. THSD1 is highly expressed in hematopoietic stem cells (HSCs) and progenitor cells, including CD34-/lowKit+Sca-1+Lin- HSCs, CD34+Kit+Sca-1+Lin- and Lin-progenitor cells, and to a lesser degree in thymic CD4-CD8- cells (1). Expression gradually decreases during T cell differentiation (1). In addition, THSD1 is widely expressed on endothelial cells, with highest expression in the lung (1). THSD1’s ligand and function are unknown, but it is postulated that THSD1 may be involved in the regulation of vasculogenesis and/or angiogenesis through its interaction with its specific ligand (1). THSD1 may function in primitive hematopoietic cells in the bone marrow niche, and through its TSP1 and Ig-like domains, may play a role in HSC homing to the niche following cell-to-cell contact to maintain hematopoietic stem cell quiescence (1).
Recombinant protein corresponding to Glu25-Asn412 from mouse Thrombospondin Type-1 Domain-containing Protein 1, fused with a 6-His-Tag at the C-terminus, expressed in mouse myeloma cell line, NS0.
Activity:Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. When 5x10e4 cells/well of are added to recombinant mouse THSD1 coated plates (30ug/ml, 100ul/well) ~50-80% will adhere after 60 minutes at 37ºC
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:Western Blot: 44.3kD migrates as an ~70-78kD protein in SDS-PAGE under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NS0 cells
Grade：Purified
Purity：≥90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤0.1EU/ug (LAL)
Form：Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile PBS.
