Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and motheaten viable” phenotypes that are severely autoimmune and immunodeficient.1 The enzyme is highly expressed in leukocyte cell types.2 SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors.3 Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity.4 SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor.5 A phosphopeptide containing the EGFR (Y992) sequence can be used to measure the activity of SHP-1 by detecting the release of phosphate.
Source: E. coli-expressed recombinant protein.
Definition: Measured by the ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site in 10mM HEPES, pH 7.5, 0.1mM EDTA, 0.1mM EGTA, 5 mg/mL BSA. Assayed for 30 minutes at 37° C.
Specific Activity: ≥ 20umoles/min/mg.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: Predicted 46kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant Human
Grade：Highly Purified
Purity：≥ 95%, as determined by SDS-PAGE and visualized by silver stain.
Form：Supplied as a liquid in 50mM Tris, 0.15M sodium chloride, 50% glycerol, 1mM DTT, pH 7.5.
