PILR-Beta (paired immunoglobulin-like type 2 receptor-beta) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-alpha and it likely arose through PILR-alpha gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-Beta and -alpha should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-Beta and -alpha show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-Beta is a 196 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a short, eight aa cytoplasmic region. The ECD shows a V-type Ig-like domain (aa 39-135), while the TM segment contains a positively-charged Lys at position # 202. This Lys is known to interact with the transmembrane signaling adaptor protein DAP12, making PILR-Beta an activating receptor. Activation of PILR-Beta through CD99 ligation induces NK cell cytotoxicity and dendritic cell secretion of NO and TNF-alpha (3). Mouse PILR-Beta is found on NK cells, neutrophils, macrophages, and monocyte-derived dendritic cells (3). Mouse PILR-Beta ECD is 44% and 75% aa identical to human and rat PILR-Beta ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-alpha (3). Evidence suggests that mouse PILR-Beta will not be active in a human system (3). Potential isoforms of PILR-Beta have been reported. One shows an alternate start site at Met14, a second shows a 21 aa substitution for the C-terminal 67 aa, and a third exhibits multiple polymorphisms for an overall aa identity of 86% (4, 5, 6).
Source: A DNA sequence encoding the extracellular domain of mouse PILR-Beta (Ala 15-Gly 180; Accession # NP_573472) was fused to a 6x histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Definition: Measured by its ability to bind rmCD99/mFc.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 19.6kD migrates as an ~ 39-44kD protein in SDS-PAGE under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant Mouse
Grade：Highly Purified
Purity：≥ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin ≤ 1EU.
Form：Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to ≥ 0.1mg/ml.
